Could not lock Volume Error 5

[Phylum]

I'm not 100% sure why, but I continue to receive access denied errors when trying to restore an img to a USB drive:

Code: Select all

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Error!
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Could not lock the volume (Code: 5)!Access is denied.


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OK   
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After clicking ok, if I try the process again I get a different error message:

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Error!
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Could not access the USB device (Code: 32)!The process cannot access the file because it is being used by another process.


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OK   
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I've tried multiple USB drives of varying sizes (2GB, 4GB, 32GB) - its the same for each. I know I've successfully done this before on this machine (back in December) so I'm not sure why it might not work now. Perhaps antivirus is interfering here?
Any suggestions/advice is appreciated!

[Alex]

It could be the Anti-Virus, but also any other process, that accesses the device. This might also be the explorer window, opened by the autostart function.